e coli atcc 8739 (ATCC)
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E Coli Atcc 8739, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 6585 article reviews
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1) Product Images from "Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity"
Article Title: Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity
Journal: Journal of Structural Biology: X
doi: 10.1016/j.yjsbx.2026.100144
Figure Legend Snippet: E. coli viability after the treatments of full-length ColIb and salt bridge variants using spot assay and CFU analysis. E. coli ATCC8739 treated with full-length WT ColIb or salt bridge variants, in the a, c absence and b, d presence of the iron chelator, 2,2′ bipyridine (100 μM), were 10-fold serially diluted and spotted. The groups treated with the protein buffer were included as a negative control. The bacterial cell viabilities treated with 0.1 nM and 0.01 nM of protein samples are compared in b . Data represent the mean ± S.D. with n = 3 independent experimental replicates. Statistical analyses were assessed using a one-way ANOVA in GraphPad Prism. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, n.s., not significant.
Techniques Used: Spot Test, Negative Control
Figure Legend Snippet: Bactericidal activity of the WT ColIb C-domain and salt bridge variants using the spot-on-lawn antimicrobial assay. Three-fold serially diluted a , b isolated ColIb C-domain and variants, and c , d full-length ColIb were spotted on the lawn of E. coli ATCC8739. The WT ColIb C-domain and variants were purified at pH 6.0 ( a , b left) and pH 4.5 ( a , b right) for spotting. The C-domain-truncated ColIb variant (TR) was pre-spotted on the plate before spotting b the ColIb C-domain and variants, and d full-length ColIb. Refer to Methods for details.
Techniques Used: Activity Assay, Isolation, Purification, Variant Assay
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