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e coli atcc 8739  (ATCC)


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    Structured Review

    ATCC e coli atcc 8739
    <t>E.</t> <t>coli</t> viability after the treatments of full-length ColIb and salt bridge variants using spot assay and CFU analysis. E. coli ATCC8739 treated with full-length WT ColIb or salt bridge variants, in the a, c absence and b, d presence of the iron chelator, 2,2′ bipyridine (100 μM), were 10-fold serially diluted and spotted. The groups treated with the protein buffer were included as a negative control. The bacterial cell viabilities treated with 0.1 nM and 0.01 nM of protein samples are compared in b . Data represent the mean ± S.D. with n = 3 independent experimental replicates. Statistical analyses were assessed using a one-way ANOVA in GraphPad Prism. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, n.s., not significant.
    E Coli Atcc 8739, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/escherichia+coli+atcc+8739/Escherichia+coli/pmc12925061-100-49-51
    Average 99 stars, based on 6585 article reviews
    e coli atcc 8739 - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity"

    Article Title: Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity

    Journal: Journal of Structural Biology: X

    doi: 10.1016/j.yjsbx.2026.100144

    E. coli viability after the treatments of full-length ColIb and salt bridge variants using spot assay and CFU analysis. E. coli ATCC8739 treated with full-length WT ColIb or salt bridge variants, in the a, c absence and b, d presence of the iron chelator, 2,2′ bipyridine (100 μM), were 10-fold serially diluted and spotted. The groups treated with the protein buffer were included as a negative control. The bacterial cell viabilities treated with 0.1 nM and 0.01 nM of protein samples are compared in b . Data represent the mean ± S.D. with n = 3 independent experimental replicates. Statistical analyses were assessed using a one-way ANOVA in GraphPad Prism. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, n.s., not significant.
    Figure Legend Snippet: E. coli viability after the treatments of full-length ColIb and salt bridge variants using spot assay and CFU analysis. E. coli ATCC8739 treated with full-length WT ColIb or salt bridge variants, in the a, c absence and b, d presence of the iron chelator, 2,2′ bipyridine (100 μM), were 10-fold serially diluted and spotted. The groups treated with the protein buffer were included as a negative control. The bacterial cell viabilities treated with 0.1 nM and 0.01 nM of protein samples are compared in b . Data represent the mean ± S.D. with n = 3 independent experimental replicates. Statistical analyses were assessed using a one-way ANOVA in GraphPad Prism. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, n.s., not significant.

    Techniques Used: Spot Test, Negative Control

    Bactericidal activity of the WT ColIb C-domain and salt bridge variants using the spot-on-lawn antimicrobial assay. Three-fold serially diluted a , b isolated ColIb C-domain and variants, and c , d full-length ColIb were spotted on the lawn of E. coli ATCC8739. The WT ColIb C-domain and variants were purified at pH 6.0 ( a , b left) and pH 4.5 ( a , b right) for spotting. The C-domain-truncated ColIb variant (TR) was pre-spotted on the plate before spotting b the ColIb C-domain and variants, and d full-length ColIb. Refer to Methods for details.
    Figure Legend Snippet: Bactericidal activity of the WT ColIb C-domain and salt bridge variants using the spot-on-lawn antimicrobial assay. Three-fold serially diluted a , b isolated ColIb C-domain and variants, and c , d full-length ColIb were spotted on the lawn of E. coli ATCC8739. The WT ColIb C-domain and variants were purified at pH 6.0 ( a , b left) and pH 4.5 ( a , b right) for spotting. The C-domain-truncated ColIb variant (TR) was pre-spotted on the plate before spotting b the ColIb C-domain and variants, and d full-length ColIb. Refer to Methods for details.

    Techniques Used: Activity Assay, Isolation, Purification, Variant Assay

    Related Articles

    other:

    Article Title: Novel Zn(II) complexes of native and modified Dendrobium hancockii polysaccharides: Synthesis, characterization, and comparative analysis of biological activities in vitro.
    Article Snippet: Native, carboxymethylated, and degraded Dendrobium hancockii polysaccharides containing Zn(II) complexes, namely PDH-Zn, CPDH-Zn, and DGPDH-Zn, were synthesized for the first time.. The three Zn(II) containing complexes and their original polysaccharides were characterized by chemical composition analysis, pre-column derivatization HPLC, FT-IR, XPS, XRD, TGA/DTG, SEM, and their biological activities were assayed in vitro.. The results showed that the complexes changed the chemical composition, sugar chain conformation and surface morphology of original polysaccharides.

    Article Title: Anti-microbial surfaces and related methods
    Article Snippet: As shown in FIG. 4A, the coating showed a greater than 99.9% reduction in Escherichia coli ATCC 8739.

    Article Title: Quaternary amine compounds with isopropylmethylphenol ester moieties as antivirals, antibacterials and antimycotics
    Article Snippet: Escherichia coli ATCC-8739 1.7×104 Cfu/ml TABLE 4 Compound MIC/μg/mL Comparative compound 2 7.8 Formula V 3.9 Formula IV 3.9 Vancomycin 78 Salmonella enterica serovar Typhimurium ATCC 14028 1.3×104 Cfu/ml TABLE 5 Compound MIC/μg/mL Comparative compound 2 7.8 Formula IV 3.9 Vancomycin 78 Escherichia coli ATCC-8739 6.6×103 Cfu/ml TABLE 6 Compound MIC/μg/mL Comparative compound 2 7.8 Formula V 3.9 Pseudomonas aeruginosa ATCC-9027 1.9×104 Cfu/ml TABLE 7 Compound MIC/μg/mL Comparative compound 2 7.8 Formula V 3.9 Escherichia coli ATCC-8739 4.1×103 Cfu/ml TABLE 8 Compound MIC/μg/mL Comparative compound 2 3.9 Formula V 1.95 Formula IV 1.95 Pseudomonas aeruginosa ATCC-9027 4.7×103 Cfu/ml TABLE 9 Compound MIC/μg/mL Comparative 1.95 compound 2 Formula IV 0.98 TABLE 10 MIC/μg/mL Comparative compound 2 Formula V Formula IV Candida 15.6 3.9-7.8 3.9-7.8 albicans ATCC 10231 TABLE 11 Comparative compound 2 Formula V Candida 15.6 3.9-7.8 albicans NCTC 885-653 TABLE 12 Compound MIC/μg/mL Comparative 1.95 compound 2 Formula V 0.98 Escherichia coli ATCC-8739 6.5×103 Cfu/ml TABLE 13 Compound MIC/μg/mL Comparative 7.8 compound 2 Formula V 3.9 Comparative 7.8 compound 3 Formula XI 3.9 Salmonella enterica serovar Typhimurium ATCC 14028 (log 4 conditions) TABLE 14 Compound MIC/mKg/mL Comparative compound 2 7.8 Formula IV 3.9 Escherichia coli ATCC-8739 (log 4 conditions) TABLE 15 Compound MIC/μg/mL Comparative compound 2 7.8 Formula V 3.9 Formula IV 3.9 Pseudomonas aeruginosa ATCC-9027 (Log 4 conditions) TABLE 16 Compound MIC/μg/mL Comparative compound 3 7.8 Formula XI 3.9 The means of MIC for different isomers (μg/ml) were tested against various fungal strains that were clinical isolate resistant or sensitive to Fluconazole and Voriconazole (resistance indicated for more than 20 fungal generations, cultivated in the presence of Fluconazole or Voriconazole).

    Article Title: Anti-microbial surfaces and related methods
    Article Snippet: As shown in FIG. 5A, the coating showed a greater than 99.9% reduction in Escherichia coli ATCC 8739.

    Article Title: Anti-microbial surfaces and related methods
    Article Snippet: As shown in FIG. 5B, the sample showed a greater than 99.9% reduction in Escherichia coli ATCC 8739.

    Article Title: Anti-microbial surfaces and related methods
    Article Snippet: As shown in FIG. 4B, the coating showed a greater than 99.9% reduction in Escherichia coli ATCC 8739.

    Activity Assay:

    Article Title: In Vitro Modeling of Mycelium Biomass Growth Kinetics of the Novel Fungicolous Species Xylaria karsticola NBIMCC 9097, with Insights into Its Antimicrobial Potential
    Article Snippet: .. Determination of the Antimicrobial Activity of the X. karsticola Extracts In the antimicrobial assays, the following strains from the collection of the Department of Microbiology and Biotechnology at the University of Food Technologies, Plovdiv, Bulgaria were used: Escherichia coli ATCC 8739, Eterococcus faecalis ATCC 19433, Salmonella enterica ssp. enterica ser. .. Enetritidis ATCC 13076, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 9027, Listeria monocytegenes ATCC 8787, Proteus vulgaris G, Klebsiella pneumonia ATCC 13883, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 11778, Wickerhamomyces anomalus, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Saccharomycodes ludwigii, and Pichia membranifaciens.

    Food & Beverages:

    Article Title: In Vitro Modeling of Mycelium Biomass Growth Kinetics of the Novel Fungicolous Species Xylaria karsticola NBIMCC 9097, with Insights into Its Antimicrobial Potential
    Article Snippet: .. Determination of the Antimicrobial Activity of the X. karsticola Extracts In the antimicrobial assays, the following strains from the collection of the Department of Microbiology and Biotechnology at the University of Food Technologies, Plovdiv, Bulgaria were used: Escherichia coli ATCC 8739, Eterococcus faecalis ATCC 19433, Salmonella enterica ssp. enterica ser. .. Enetritidis ATCC 13076, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 9027, Listeria monocytegenes ATCC 8787, Proteus vulgaris G, Klebsiella pneumonia ATCC 13883, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 11778, Wickerhamomyces anomalus, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Saccharomycodes ludwigii, and Pichia membranifaciens.

    Virus:

    Article Title: Iodine-Based Coordination Compounds: A Strategy Toward Antibiotic Potentiation
    Article Snippet: The bacterial and fungal test strains were obtained from the American Type Culture Collection (ATCC) through the official distributor for Central Asia LGC Standards (Poland). .. Both reference (museum) and clinical strains, including sensitive and multidrug-resistant variants, were employed as follows: Staphylococcus aureus ATCC 6538-P (museum, sensitive strain) Staphylococcus aureus ATCC 333591 (museum, multidrug-resistant strain) Escherichia coli ATCC 8739 (museum, sensitive strain) Escherichia coli ATCC BAA-2523 (museum, multidrug-resistant strain) Pseudomonas aeruginosa ATCC 9027 (museum, sensitive strain) Pseudomonas aeruginosa TA2 (clinical, multidrug-resistant strain) Klebsiella pneumoniae ATCC 10031 (museum, sensitive strain) Klebsiella pneumoniae ATCC 2524 (museum, multidrug-resistant strain) Salmonella enterica ATCC 14028 (museum, sensitive strain) Salmonella enterica ATCC 35988 (museum, multidrug-resistant strain) Enterococcus hirae ATCC 10541 (museum, sensitive strain) Enterococcus faecalis ATCC 51575 (museum, multidrug-resistant strain) Candida albicans ATCC 10231 (museum, sensitive strain) Candida albicans SCID PHRX 1-2019 (clinical, multidrug-resistant strain) Virus strains—Influenza A virus A/Swine/Iowa/15/30 (H1N1) and Herpes Simplex Virus type 1 (strain “Victory”), obtained from the Laboratory of Viral Ecology, Institute of Virology and Microbiology, Committee of Science, MES RK. ..



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    E. coli viability after the treatments of full-length ColIb and salt bridge variants using spot assay and CFU analysis. E. coli ATCC8739 treated with full-length WT ColIb or salt bridge variants, in the a, c absence and b, d presence of the iron chelator, 2,2′ bipyridine (100 μM), were 10-fold serially diluted and spotted. The groups treated with the protein buffer were included as a negative control. The bacterial cell viabilities treated with 0.1 nM and 0.01 nM of protein samples are compared in b . Data represent the mean ± S.D. with n = 3 independent experimental replicates. Statistical analyses were assessed using a one-way ANOVA in GraphPad Prism. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, n.s., not significant.

    Journal: Journal of Structural Biology: X

    Article Title: Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity

    doi: 10.1016/j.yjsbx.2026.100144

    Figure Lengend Snippet: E. coli viability after the treatments of full-length ColIb and salt bridge variants using spot assay and CFU analysis. E. coli ATCC8739 treated with full-length WT ColIb or salt bridge variants, in the a, c absence and b, d presence of the iron chelator, 2,2′ bipyridine (100 μM), were 10-fold serially diluted and spotted. The groups treated with the protein buffer were included as a negative control. The bacterial cell viabilities treated with 0.1 nM and 0.01 nM of protein samples are compared in b . Data represent the mean ± S.D. with n = 3 independent experimental replicates. Statistical analyses were assessed using a one-way ANOVA in GraphPad Prism. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, n.s., not significant.

    Article Snippet: Since the above experimental results demonstrated that disrupting the electrostatic interactions in the C-domain leads to a more loosely packed tertiary structure, which may reduce the unfolding energy required for translocation through CirA and facilitate membrane insertion, we sought to characterize the antibacterial activity by monitoring the viability of E. coli ATCC 8739 treated with full-length ColIb and its salt bridge variants ( , ).

    Techniques: Spot Test, Negative Control

    Bactericidal activity of the WT ColIb C-domain and salt bridge variants using the spot-on-lawn antimicrobial assay. Three-fold serially diluted a , b isolated ColIb C-domain and variants, and c , d full-length ColIb were spotted on the lawn of E. coli ATCC8739. The WT ColIb C-domain and variants were purified at pH 6.0 ( a , b left) and pH 4.5 ( a , b right) for spotting. The C-domain-truncated ColIb variant (TR) was pre-spotted on the plate before spotting b the ColIb C-domain and variants, and d full-length ColIb. Refer to Methods for details.

    Journal: Journal of Structural Biology: X

    Article Title: Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity

    doi: 10.1016/j.yjsbx.2026.100144

    Figure Lengend Snippet: Bactericidal activity of the WT ColIb C-domain and salt bridge variants using the spot-on-lawn antimicrobial assay. Three-fold serially diluted a , b isolated ColIb C-domain and variants, and c , d full-length ColIb were spotted on the lawn of E. coli ATCC8739. The WT ColIb C-domain and variants were purified at pH 6.0 ( a , b left) and pH 4.5 ( a , b right) for spotting. The C-domain-truncated ColIb variant (TR) was pre-spotted on the plate before spotting b the ColIb C-domain and variants, and d full-length ColIb. Refer to Methods for details.

    Article Snippet: Since the above experimental results demonstrated that disrupting the electrostatic interactions in the C-domain leads to a more loosely packed tertiary structure, which may reduce the unfolding energy required for translocation through CirA and facilitate membrane insertion, we sought to characterize the antibacterial activity by monitoring the viability of E. coli ATCC 8739 treated with full-length ColIb and its salt bridge variants ( , ).

    Techniques: Activity Assay, Isolation, Purification, Variant Assay